Featured White
Papers
Designing a Reversed-Phase Column for Polar Compound Retention , Waters
Fully aqueous-compatible packings have been made by combining properly tailored silica substrates with a novel, new, T3-bonding process, designed to create bonded phases with enhanced stability in acidic solutions and excellent peak shape. These columns set a new performance standard for polar compound retention and selectivity in HPLC and UPLC® separations.
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Effect of Pore Sizes in UPLC® Peptide Mapping , Waters
The enhanced chromatographic resolution associated with UltraPerformance Liquid Chromatography (UPLC) has been demonstrated for peptide mapping. In general, this technique improves resolution by a factor of 3 or more. While many chromatographers prefer the use of large pore material (i.e. 300 Å) for peptide separation, the use of 130 Å packing material may be advantageous for some samples. For more information, read the full application note.
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UPLC® for the Analysis of Synthetic Peptides and for the Development of Isolation Strategies , Waters
Peptides are used in many areas of basic research and can also be utilized as important biotherapeutic agents. The Waters UPLC® Peptide Analysis Solution was first described as a tool for peptide mapping. It is also a proven technology for synthetic peptide applications. The inherently high resolving power of UPLC® technology ensures that contaminants can be effectively separated in relatively rapid runs for various analytical or method development applications. For more information, read the full application note.
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Topics in Solid-Phase Extraction. Part 1. Ion Suppression in LC/MS Analysis: A Review. Strategies for its elimination by well-designed, multidimensional solid-phase extraction [SPE] protocols and methods for its quantitative assessment , Waters
Ion suppression — a common phenomenon in the LC/MS analysis of complex samples — can be effectively eliminated by good solid-phase extraction methods. Today, with tools such as SPE, coupled with the availability of methods for the quantitative assessment of ion suppression, significant errors in LC/MS analysis due to ion suppression are avoidable and inexcusable.
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Post-Column Derivatization , Pickering Labs
The majority of pharmaceuticals are polar, nonvolatile or thermally labile molecules that are amenable to HPLC separation. Factors that increase analyte signal strength will lower the limit of detection and quantitation often permits simpler and more efficient sample preparation.
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Transfer of the USP Assay For Simvastatin TO UPLC® , Waters
The Uniformity of Content Assay for simvastatin, a lipid-lowering agent that is derived synthetically from a fermentation product of
Aspergillus terreus, is accomplished using high performance liquid chromatography (HPLC) with ultraviolet detection (UV). The faster that this assay can be performed, the faster the finished product can be released and revenue realized. In this application note, we show how the HPLC assay for
simvastatin has been transferred to the UPLC assay, which is then compared to the USP assay criteria for performance and quality.
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Acquity UPLC® Assay of Amoxicillan and Potassium Clavulanate from an Oral Suspension , Waters
The migration and consolidation of legacy methods to the Waters ACQUITY UPLC System is a major focus for companies adopting UltraPerformance LC® (UPLC®) technology. Those companies that invest in the process are rewarded with significant savings in analysis times and operational costs while conserving or improving overall chromatographic performance. This application note summarizes the results of a simple migration of a USP-based HPLC assay of amoxicillin and potassium clavulanate from an oral suspension, an established HPLC method, that can be simply migrated to a UPLC-based method.
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Enabling Significant Improvements for Peptide Mapping with UPLC™ , Waters
Peptide mapping continues to be the preferred technique for the comprehensive characterization of biopharmaceutical products. Its applications include: the identification of proteins based on the elution pattern of peptide fragments, the determination of post-translational modifications, the confirmation of genetic stability, and the analysis of protein sequences when interfaced to a mass spectrometer.
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Quantitative Aspects of UPLC® Peptide Mapping , Waters
Ultra Performance Liquid Chromatography (UPLC®) has demonstrated significant advantages compared to HPLC for peptide mapping. UPLC gives increased resolution, higher sensitivity, excellent peak shapes for glycopeptides, and the potential to increase throughput. In this application note, we focus on the quantitative aspects of UPLC peptide mapping with UV detection.
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Transitioning Existing LC Methods To New Technologies , Waters
Laboratories
making investments in new LC platforms must take into account their
ability to run legacy methods, while also preparing themselves for
future methods that will employ new column technologies. This white
paper discusses the importance of choosing a Liquid Chromatography
platform for 2012.
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