White Papers

Designing a Reversed-Phase Column for Polar Compound Retention , Waters
Fully aqueous-compatible packings have been made by combining properly tailored silica substrates with a novel, new, T3-bonding process, designed to create bonded phases with enhanced stability in acidic solutions and excellent peak shape. These columns set a new performance standard for polar compound retention and selectivity in HPLC and UPLC® separations.
Read more of this white paper.

Effect of Pore Sizes in UPLC® Peptide Mapping , Waters
The enhanced chromatographic resolution associated with UltraPerformance Liquid Chromatography (UPLC) has been  demonstrated for peptide mapping. In general, this technique improves resolution  by a factor of 3 or more. While many  chromatographers prefer the use of large pore material (i.e. 300 Å) for peptide separation,  the use of 130 Å packing material may be advantageous for some samples.  For more information, read the full application note.
Read more of this white paper.

UPLC® for the Analysis of Synthetic Peptides and for the Development of Isolation Strategies , Waters
Peptides are used in many areas of basic research and can also be utilized as important biotherapeutic agents. The Waters UPLC® Peptide Analysis Solution was first described as a tool for peptide mapping. It is also a proven technology for synthetic peptide applications. The inherently high resolving power of UPLC® technology ensures that contaminants can be effectively separated in relatively rapid runs for various analytical or method development applications. For more information, read the full application note.
Read more of this white paper.

Topics in Solid-Phase Extraction. Part 1. Ion Suppression in LC/MS Analysis: A Review. Strategies for its elimination by well-designed, multidimensional solid-phase extraction [SPE] protocols and methods for its quantitative assessment , Waters
Ion suppression — a common phenomenon in the LC/MS analysis of complex samples — can be effectively eliminated by good solid-phase extraction methods. Today, with tools such as SPE, coupled with the availability of methods for the quantitative assessment of ion suppression, significant errors in LC/MS analysis due to ion suppression are avoidable and inexcusable.
Read more of this white paper.

Post-Column Derivatization , Pickering Labs
The majority of pharmaceuticals are polar, nonvolatile or thermally labile molecules that are amenable to HPLC separation. Factors that increase analyte signal strength will lower the limit of detection and quantitation often permits simpler and more efficient sample preparation.
Read more of this white paper.

Transfer of the USP Assay For Simvastatin TO UPLC® , Waters
The Uniformity of Content Assay for simvastatin, a lipid-lowering agent that is derived synthetically from a fermentation product of Aspergillus terreus, is accomplished using high performance liquid chromatography (HPLC) with ultraviolet detection (UV). The faster that this assay can be performed, the faster the finished product can be released and revenue realized. In this application note, we show how the HPLC assay for simvastatin has been transferred to the UPLC assay, which is then compared to the USP assay criteria for performance and quality.
Read more of this white paper.

Acquity UPLC® Assay of Amoxicillan and Potassium Clavulanate from an Oral Suspension , Waters
The migration and consolidation of legacy methods to the Waters ACQUITY UPLC System is a major focus for companies adopting UltraPerformance LC® (UPLC®) technology. Those companies that invest in the process are rewarded with significant savings in analysis times and operational costs while conserving or improving overall chromatographic performance. This application note summarizes the results of a simple migration of a USP-based HPLC assay of amoxicillin and potassium clavulanate from an oral suspension, an established HPLC method, that can be simply migrated to a UPLC-based method.
Read more of this white paper.

Enabling Significant Improvements for Peptide Mapping with UPLC™ , Waters
Peptide mapping continues to be the preferred technique for the comprehensive characterization of biopharmaceutical products. Its applications include: the identification of proteins based on the elution pattern of peptide fragments, the determination of post-translational modifications, the confirmation of genetic stability, and the analysis of protein sequences when interfaced to a mass spectrometer.
Read more of this white paper.

Quantitative Aspects of UPLC® Peptide Mapping , Waters
Ultra Performance Liquid Chromatography (UPLC®) has demonstrated significant advantages compared to HPLC for peptide mapping. UPLC gives increased resolution, higher sensitivity, excellent peak shapes for glycopeptides, and the potential to increase throughput. In this application note, we focus on the quantitative aspects of UPLC peptide mapping with UV detection.
Read more of this white paper.

Transitioning Existing LC Methods To New Technologies , Waters
Laboratories making investments in new LC platforms must take into account their ability to run legacy methods, while also preparing themselves for future methods that will employ new column technologies. This white paper discusses the importance of choosing a Liquid Chromatography platform for 2012.
Read more of this white paper.

The Time Is Right for Labs to Embrace the Principles of Industrial Automation , John Dulchinos of Adept Technologies and Peter Massaro of Protedyne Corporation
Industrial automation technology has the potential to revolutionize conventional laboratory sample processing.  Discover how industrial automation principals can help labs improve efficiencies, advance performance, minimize risk and maintain scientific integrity.
Read more of this white paper.

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA) , Sigma-Aldrich
Preserved samples from medical, forensic, museum and other archival collections represent a rich source of study material, much of it meticulously collected, characterized and preserved through many decades of work by experts in the field. While the majority of this material was originally collected and preserved with morphological studies in mind, the advent of molecular genetic techniques have caused many scientist to eye archival collections as a precious source of biological data.
Read more of this white paper.

Delivering Interactive Parallel Computing Power to the Desktop , Interactive Supercomputing
The emergence of new software tools and cost-effective parallel computers are ushering in a new era for scientists and engineers working on large and complex problems. Familiar desktop tools can be used to develop new algorithms and applications, working with much larger datasets, and transparently tapping into the computing power and large memory of parallel servers.
Read more of this white paper.

Waters Optimum Bed Density [OBD™] Columns: Enabling Technology for Laboratory-Scale Isolation and Purification, Waters OBD™
Discover how Waters OBD™ Prep columns bring dramatic improvement in stability, lifetime, efficiency, peak shape, and reproducibility results from continuing investigation of, and innovation in, the design of particles, columns, and packing processes.
Read more of this white paper.

Ultra Fast Mutation Discovery and Genotyping, Idaho Technology
Listen to a streaming introduction of this new technology and download the technical whitepaper.
Read more of this white paper.

Innovations that are changing the world - and peoples' lives - every day, IBM
Learn how thousands of scientists are conducting genetic research through TGen, a Phoenix-based biomedical research firm. Read about the power of innovation and how IBM, with its Business Partners, is creating some of the most exciting and innovative solutions in the world.
Read more of this white paper

Achieving Accurate and Reliable Resistance Measurements in Low Power and Low Voltage Applications, Keithley
Low voltage measurements are often associated with resistance measurements of highly conductive semiconductor materials and devices. These tests normally involve sourcing a known current, measuring the resulting voltage, and calculating the resistance using Ohm's Law. Because of the DUT's inherent low resistance, the resulting voltage will be very small and great care needs to be taken to reduce offset voltage and noise, which can normally be ignored when measuring higher signal levels.
Read more of this white paper.

Measuring Electrical Performance in Nanodevices and Detecting Single Electron Transfer at Room Temperature, Agilent Technologies
Exotic carbon nanotube(CNT) structures have generated a great deal of interest in the scientific community. There are many potential applications for CNTs, but much of the current focus in nanotechnology research is centered on electrical component structures, such as the CNT field effect transistor (FET) and the CNT single electron transistor (SET).
Read more of this white paper